Home > Sandwich ELISA Kits > Rat Platelet Derived Growth Factor Subunit B (PDGF-B) ELISA Kit

Product Name:

Rat Platelet Derived Growth Factor Subunit B (PDGF-B) ELISA Kit

Cat.#:

JL21402

Regulatory Status:

RUO

Alternative:

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

12.75pg/mL

Specificity:

This kit recognizes Rat PDGF-B in samples. No significant cross-reactivity or interference between Rat PDGF-B and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PDGF-B

Platelet-derived growth factor subunit B is a protein that is encoded by the PDGFB gene.The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha (PDGFA) polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.34	1.88	1.37	1.11	0.79	0.43	0.23	0.08
Corrected OD	2.26	1.8	1.29	1.03	0.71	0.35	0.15	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat PDGF-B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Rat PDGF-B and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98